Please use this identifier to cite or link to this item: http://idr.niser.ac.in:8080/jspui/handle/123456789/1173
Title: Thermosensitive ion channel TRPV1 is endogenously expressed in the sperm of a fresh water teleost fish (Labeo rohita) and regulates sperm motility
Authors: Majhi, Rakesh Kumar
Kumar, Ashutosh
Yadav, Manoj
Swain, Nirlipta
Kumari, Shikha
Nayak, Tapas Kumar
Chattopadhyay, Subhasis
Goswami, Chandan
Keywords: Labeo rohita
Ca2+ channels
Capsaicin
NADA
TRPV1
Vertebrate evolution
sperm cells
sperm motility
teleost fish
Issue Date: 2-Aug-2013
Publisher: Channels
Citation: Majhi, R. K., Kumar, A., Yadav, M., Swain, N., Kumari, S., Saha, A., … Goswami, C. (2013). Thermosensitive ion channel TRPV1 is endogenously expressed in the sperm of a fresh water teleost fish (Labeo rohita) and regulates sperm motility. Channels (Austin, Tex.), 7(6), 483–492.
Abstract: Sperm cells exhibit extremely high sensitivity in response to slight changes in temperature, osmotic pressure and/or presence of various chemical stimuli. In most cases throughout the evolution, these physico-chemical stimuli trigger Ca2+-signaling and subsequently alter structure, cellular function, motility and survival of the sperm cells. Few reports have recently demonstrated the presence of Transient Receptor Potential (TRP) channels in the sperm cells from higher eukaryotes, mainly from higher mammals. In this work, we have explored if the sperm cells from lower vertebrates can also have thermo-sensitive TRP channels. In this paper, we demonstrate the endogenous presence of one specific thermo-sensitive ion channel, namely Transient Receptor Potential Vanilloid family member sub type 1 (TRPV1) in the sperm cells collected from fresh water teleost fish, Labeo rohita. By using western blot analysis, fluorescence assisted cell sorting (FACS) and confocal microscopy; we confirm the presence of this non-selective cation channel. Activation of TRPV1 by an endogenous activator NADA significantly increases the quality as well as the duration of fish sperm movement. The sperm cell specific expression of TRPV1 matches well with our in silico sequence analysis. The results demonstrate that TRPV1 gene is conserved in various fishes, ranging from 1–3 in copy number, and it originated by fish-specific duplication events within the last 320 million years (MY). To the best of our knowledge, this is the first report demonstrating the presence of any thermo-sensitive TRP channels in the sperm cells of early vertebrates as well as of aquatic animals, which undergo external fertilization in fresh water. This observation may have implications in the aquaculture, breeding of several fresh water and marine fish species and cryopreservation of fish sperms.
URI: https://doi.org/10.4161/chan.25793
http://idr.niser.ac.in:8080/jspui/handle/123456789/1173
Appears in Collections:Journal Papers

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